Fabrication And Characterisation Of Chitosan-Strontium Films For Biomedical Applications
Main Article Content
Abstract
BACKGROUND: Chitosan-strontium is a promising nano-material for bone tissue engineering. It improves biocompatibility and bone binding ability. Chitosan is a partially deacetylated version of chitin, is regarded a good stabilizer to be efficiently capped with metal oxide nanoparticles due to its unique structural features.Strontium oxide is recognized as an excellent catalyst for transesterification and is one of the most promising heterogeneous base catalysts among processable alkaline earth metal oxides.
AIM: To fabricate and characterize Chitosan- strontium films for biomedical applications.
MATERIALS AND METHODS: Chitosan complexed with strontium using simple complexation method. The chitosan-Sr complex is formed by the interaction of amino groups and hydroxyl groups of chitosan with strontium ions. It reduces the Sr ions into Sr nanoparticles due to the intrinsic reduction property.
RESULT: Chitosan-strontium complex was found to be non toxic to human osteoblastic cells. It had no effects on the metabolic activity (MTT Assay) and cell membrane integrity (LDH assay). The complex also promoted osteoblasts differentiation. It improved calcium deposition by the osteoblasts (Alizarin red staining). Therefore, the complex can be explored for bone tissue engineering applications.
CONCLUSION: Our results implicated that Chitosan-strontium films can be considered as a promising cost-effective nano- material for human bone regenerative applications...