Silver and iron nanoparticles studies on Torenia crustacea (L.) Chamisso. & Schltendal.

Abstract

Nature has devised various processes for the synthesis of nano and micro length scaled inorganic materials which have contributed to the development of relatively new and largely unexplored area of research based on the biosynthesis of nanoparticles. Green synthesis of silver and iron nanoparticles was based on colour developed in the solution at the time of reaction and the colour intensity depends on peak in the UV-visible spectroscopy. FTIR analysis of silver nanoparticles confirms the presence of eight functional groups such as alkenes, aromatics, alkyl halides, esters, ketones, amides, aromatics and misc with frequency ranges (cm^-1). SEM analysis of T. crustacea brown coloured sample shows the presence of clustered spherical shaped, non-uniform distribution of silver nanoparticles and the TEM analysis enumerates many spherical shaped non-uniform distribution of silver nanopaticles of different sizes such as 7.98 nm, 12.34 nm, 11.87 nm, 13.56 nm and 15.92 nm having a mean size of about 16.09 nm lies in the nano range. FTIR analysis for iron nanoparticles of the ethanolic extract of the powdered whole plant of T. crustacea is used for identifying the functional groups of active components such as alkynes, alkanes, amides, misc, alkenes, carboxylic acids, aromatics and alkyl halides having the frequency range (cm-¹). SEM analysis having mostly clustered spherical shaped iron nanoparticles and the TEM images showed that spherical shaped iron nanoparticles of non-uniform distribution with mean size of about 16.07nm. Anti-microbial activity of the whole plant silver and iron coated extract of T. crustacea was performed by using five bacterial strains such as Escherichia coli, Klebsiella pneumonia, Bacillus subtilis, Staphylococcus aureus, Lactobacillus acidophilus and three fungal strains such as Aspergillus niger, Aspergillus flavus and Pencillium notatum.