Development Of Analytical Method And Validation For Estimation Of Quercetin And Monoammonium Glycyrrhizinate In Their Formulation

Abstract

A unique, rapid, straightforward, and precise UV spectrophotometric approach for the simultaneous quantification of quercetin and Monoammonium glycyrrhizinate was developed and validated. Ethanol served as a solvent while simultaneous equations were employed to quantify quercetin and Monoammonium glycyrrhizinate at wavelengths of 377 nm and 248 nm, respectively. The concentration ranges for quercetin and Monoammonium glycyrrhizinate were 2 to10 μg/ml and 10 to 60 μg/ml, respectively, adhering to Beer's law. This methodology was validated in accordance with ICH requirements on accuracy, linearity, precision, LOD, and LOQ. Given that the %RSD was below 2, this method was deemed accurate. The proposed approach for the routine analysis of quercetin and monoammonium glycyrrhizinate in gel formulation has demonstrated efficiency, specificity, accuracy, and precision.